The Childhood Cancer Repository, powered by Alex's Lemonade Stand Foundation, is a resource laboratory of the Children's Oncology Group. The laboratory is located at Texas Tech University Health Sciences Center in Lubbock, TX and is under the direction of C Patrick Reynolds, MD PhD. The repository is generously supported by Alex's Lemonade Stand Foundation. Support for the animal facilities used by the repository is provided by the Cancer Prevention & Research Institute of Texas. Support for genomic and biological characterization of cell lines and PDXs in the repository comes from the National Cancer Institute and the Department of Defense.
With the exception of legacy cell lines established many years ago, all cell lines and PDXs banked in cccells.org are obtained via clinical protocols (mostly COG) with informed consent/assent. Patient confidentiality is a prime concern and cell lines and PDXs receive designators that enables linkage to key data but not directly to any patient. The one exception to this are those cell lines and PDXs established from patients at post-mortem, which (if requested by the parents) are provided a unique name of the parents' choosing.
The goal of the repository is to provide validated cell lines and PDXs from childhood cancers to all investigators seeking to carry out biological and pre-clinical therapeutic studies of childhood cancer. Every cell line or PDX provided by the repository is validated as to patient of origin by short tandem repeat (STR) assay, and to be free of mycoplasma and EBV. PDXs are validated to be free of a panel of viral pathogens as required by most animal use committees. Cell lines and PDXs are validated to be able to grow continuously and be sub-passable for additional studies. The repository maintains a searchable STR database to facilitate validation of cell line and PDX molecular identity by any investigator. Thus, the repository enables all investigators studying childhood cancer to share the same validated models so that data obtained are robust and can be compared between laboratories.
Childhood Cancer Repository Staff
|Director:||C Patrick Reynolds, MD PhD|
|Lab Managers:||Jonas Nance
|Our Team:||Wan Hsi (Wendy) Chen
Childhood Cancer Repository Laboratory Facilities
The Childhood Cancer Repository has a dedicated cell culture laboratory at TTUHSC and is set up with a separate cell line initiation and cell line expansion/banking laboratories. These labs are positive pressure to the central personnel housing and bench laboratory, and the latter positive pressure to hallways. The labs are each equipped with 4 incubators (O2 control capable), 3 biosafety cabinets, centrifuges, microscopes, and digital photomicroscopy.
A molecular core lab supports the biorepository and houses an ABI sequencer optimized for short tandem repeat assays and a TaqMan quantitative RT-PCR system equipped for Taqman low density arrays (TLDA). For characterizing lines or if sorting is necessary, an adjacent core lab has a BD LSR-II flow cytometer and a FACSaria cell sorter.
Cryopreservation facilities for the biorepository are robust. Storage is in MVE 1800 Series 190F LN2 vapor freezers which are fed from an external dedicated 7000 gallon LN2 source via vacuum-jacketed lines. Each MVE 190F freezer can hold 70,800 cryovials, and with 5 freezers, the biorepository has a capacity of 354,000 cryovials. Space in the biorepository and in place LN2 feed connections allows for expansion to double the biorepository. The lab also has 10-80° freezers for tissue and fetal bovine serum storage. All freezers are constantly remote monitored with web access and email notification of alarms.
Above right, LN2 freezers.
Left, LN2 supply.
Right, Lab layout (downloadable PDF map).