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TaqMan RT-PCR Primer/Probe Sets Used for Study of Sphingosine Kinase

Youngleem Kim, Zesheng Wan, C. Patrick Reynolds
Developmental Therapeutics Program
USC-CHLA Institute for Pediatric Clinical Research

The primers were designed using Primer Express V 1.5 software from Applied Biosystems, based on sequences from GenBank/EMBL/DDBJ. Use of thsese primer probe sets will be described in a manuscript in preparation.


human sphingosine kinase 1 (SPHK1: GenBank AF200328)
-forward primer: 5´-GTCACGTGCAGCCCCTTT-3´;
-reverse primer: 5´-CGCGCGTGGTTCCG-3´
-probe: 5´-CTTCACGCTGATGCTCACTGAGCG-3´

human sphingosine kinase 2 gene (SPHK2 GenBank AF245447)
-forward primer: 5´-TGCTCCATGAGGTGCTGAAC-3´
-reverse primer: 5´-GCCCACAGGCATCTTCACA-3´
-probe: 5´-TTCCTCCCAGTCAGGGCGATCTAGGA-3´

human bcl-2 gene (GenBank M14745)
-forward primer: 5´-CATGTGTGTGAGAGCGTCAA-3´
-reverse primer: 5´-AACAGAGGCCGCATGCTG-3´
-probe: 5´-CCTGGTGGACAACATCGCCCTGT-3´

Glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH GeneBank M33197)
-forward primer: 5´-CAACTACATGGTTTACATGTTCCAATATG-3´
-reverse primer: 5´-GGGATCTCGCTCCTGGAAG -3´
-probe: 5´-CGTTCTCAGCCTTGACGGTGC CA -3´

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